Anti-RAB35 Antibody (A16047)

Name: Anti-RAB35 Antibody
Brand: Antibodies.com
SKU: A16047
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to RAB35 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-RAB35 Antibody

Description

Rabbit polyclonal antibody to RAB35.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-201 of human RAB35 (NP_006852.1).

Sequence

KRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GTP binding protein RAY, GTP-binding protein RAY, H ray, OTTHUMP00000240256, OTTHUMP00000240257, OTTHUMP00000240258, OTTHUMP00000240259, RAB1C, RAB35 member RAS oncogene family, RAB35, member RAS oncogene family, RAB35_HUMAN, Ras related protein Rab 1C, Ras related protein rab 1c (GTP binding protein ray), Ras related protein Rab35, Ras-related protein Rab-1C, Ras-related protein Rab-35, RAY

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-RAB35 Antibody (A16047)

Western blot analysis of extracts of various cell lines, using Anti-RAB35 Antibody (A16047) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-RAB35 Antibody (A16047)

Western blot analysis of extracts of HeLa cells, using Anti-RAB35 Antibody (A16047) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-RAB35 Antibody (A16047)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-RAB35 Antibody (A16047) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-RAB35 Antibody (A16047)

Immunofluorescence analysis of PC-12 cells using Anti-RAB35 Antibody (A16047) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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