Anti-QKI Antibody [S147-6] (A304994)

Name: Anti-QKI Antibody [S147-6]
Brand: Antibodies.com
SKU: A304994
Price: 500.0 USD
Availability: InStock

Enlarge Image

$500

Mouse monoclonal [S147-6] antibody to QKI for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-QKI Antibody [S147-6]

Description

Mouse monoclonal [S147-6] antibody to QKI.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b..

Host

Mouse

Clonality

Monoclonal

Clone ID

S147-6

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~36-38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp586I0923, HKQ, Homolog of mouse quaking QKI KH domain RNA binding protein, Hqk, HQK1, HqkI, OTTHUMP00000017581, OTTHUMP00000017582, OTTHUMP00000017583, Protein quaking, QK, QK1, QK3, QKI1, QKI_HUMAN, Quaking homolog, Quaking homolog KH domain RNA binding, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-QKI Antibody [S147-6] (A304994)

Western blot analysis of rat brain membrane showing detection of 36-38 kDa QKI (pan) protein using Anti-QKI Antibody [S147-6] (A304994) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 36-38 kDa.

Enlarge Image

Figure 2: Immunofluorescence - Anti-QKI Antibody [S147-6] (A304994)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-QKI Antibody [S147-6] (A304994), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) QKI (pan) Antibody. (D) Composite.

Enlarge Image

Figure 3: Immunofluorescence - Anti-QKI Antibody [S147-6] (A304994)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-QKI Antibody [S147-6] (A304994), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI (pan) Antibody (D) Composite.

Publishing research using Anti-QKI Antibody [S147-6] (A304994)? Please let us know so that we can list the citation on this page.