Anti-PYK2 (phospho Tyr402) Antibody (A16518)

Name: Anti-PYK2 (phospho Tyr402) Antibody
Brand: Antibodies.com
SKU: A16518
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to PYK2 (phospho Tyr402) for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-PYK2 (phospho Tyr402) Antibody

Description

Rabbit polyclonal antibody to PYK2 (phospho Tyr402).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y402 of human PTK2B (NP_004094.3).

Sequence

DIYAE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

116 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CADTK, CAK-beta, CAKB, CAKbeta, Calcium regulated non receptor proline rich tyrosine kinase, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, E430023O05Rik, EC 2.7.10.2, FADK 2, FADK2, FAK2, FAK2_HUMAN, Focal adhesion kinase 2, MGC124628, PKB, Proline-rich tyrosine kinase 2, Protein kinase B, Protein Tyrosine Kinase 2 Beta, Protein-tyrosine kinase 2-beta, PTK, PTK2B, PTK2B protein tyrosine kinase 2 beta, RAFTK, RAFTK2, Related adhesion focal tyrosine kinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PYK2 (phospho Tyr402) Antibody (A16518)

Western blot analysis of extracts of Jurkat cells, using Anti-PYK2 (phospho Tyr402) Antibody (A16518) at 1:1,000 dilution. Jurkat cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-PYK2 (phospho Tyr402) Antibody (A16518)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-PYK2 (phospho Tyr402) Antibody (A16518). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.