Anti-PYK2 (phospho Tyr402) Antibody (A16518)

Name: Anti-PYK2 (phospho Tyr402) Antibody
Brand: Antibodies.com
SKU: A16518
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to PYK2 (phospho Tyr402) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PYK2 (phospho Tyr402) Antibody

Description

Rabbit polyclonal antibody to PYK2 (phospho Tyr402).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y402 of human PTK2B (NP_004094.3).

Sequence

DIYAE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

116 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CADTK, CAK-beta, CAKB, Calcium-dependent tyrosine kinase, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, FADK 2, FAK2, Focal adhesion kinase 2, Proline-rich tyrosine kinase 2, Protein-tyrosine kinase 2-beta, PTK2B, RAFTK, Related adhesion focal tyrosine kinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PYK2 (phospho Tyr402) Antibody (A16518)

Western blot analysis of extracts of Jurkat cells, using Anti-PYK2 (phospho Tyr402) Antibody (A16518) at 1:1,000 dilution. Jurkat cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-PYK2 (phospho Tyr402) Antibody (A16518)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-PYK2 (phospho Tyr402) Antibody (A16518). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.