Anti-PYGL Antibody (A15341)

Name: Anti-PYGL Antibody
Brand: Antibodies.com
SKU: A15341
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PYGL for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PYGL Antibody

Description

Rabbit polyclonal antibody to PYGL.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 690-847 of human PYGL (NP_002854.3).

Sequence

IGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

97 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Glycogen phosphorylase, Glycogen phosphorylase L, Glycogen phosphorylase liver, Glycogen phosphorylase liver form, GSD6, Hers disease, glycogen storage disease type VI, liver form, OTTHUMP00000233649, OTTHUMP00000233651, Phosphorylase glycogen liver, PYGL_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PYGL Antibody (A15341)

Western blot analysis of extracts of various cell lines, using Anti-PYGL Antibody (A15341) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-PYGL Antibody (A15341)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-PYGL Antibody (A15341) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-PYGL Antibody (A15341)

Immunofluorescence analysis of HeLa cells using Anti-PYGL Antibody (A15341) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PYGL Antibody (A15341)

Immunofluorescence analysis of HepG2 cells using Anti-PYGL Antibody (A15341) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).