This antibody recognises the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kDa pro-apoptotic member of the BCL-2 family.Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Rabbit anti PUMA antibody is expected to recognize the canonical isoform 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

Applications

WB, IHC-P

Dilutions

IHC-P 1: 10 µg/ml, WB: 2 µg/ml - 4 µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide sequence ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.

Sequence

ARARQEGSSPEPVEG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Concentration

1 mg/ml

Molecular Weight

Approximately 23 kDa in K562 cell lysates.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

General Notes

Rabbit anti PUMA antibody recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kDa pro-apoptotic member of the BCL-2 family.Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53 (Follis et al. 2013). Four isoforms of PUMA have been described due to alternative splicing. Rabbit anti PUMA antibody is expected to recognize the canonical isoform 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

Synonyms

BBC 3, Bbc3, BBC3_HUMAN, BCL 2 binding component 3, Bcl-2-binding component 3, BCL2 binding component 3, JFY 1, JFY-1, JFY1, p53 up regulated modulator of apoptosis, p53 up-regulated modulator of apoptosis, p53 Upregulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

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