Anti-Patched / PTCH1 Antibody (A11198)

Name: Anti-Patched / PTCH1 Antibody
Brand: Antibodies.com
SKU: A11198

Enlarge Image

This product is discontinued

Anti-Patched / PTCH1 Antibody (A11198) has been discontinued and is no longer available.

View all Primary Antibodies.

Specifications

Name

Anti-Patched / PTCH1 Antibody

Description

Rabbit polyclonal antibody to Patched / PTCH1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1214-1312 of human PTCH1 (NP_000255.2).

Sequence

THSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

161 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

A230106A15Rik, BCNS, FLJ26746, FLJ42602, Holoprosencephaly 7, HPE7, mes, NBCCS, OTTHUMP00000021709, OTTHUMP00000021710, Patched, Patched (Drosophila) homolog, Patched 1, Patched homolog (Drosophila), Patched homolog 1, Patched homolog 1 (Drosophila), Patched protein homolog 1, Protein patched homolog 1, PTC, PTC1, PTC1_HUMAN, PTCH, PTCH protein, PTCH protein +12b, PTCH protein +4', PTCH protein -10, ptch1, PTCH1 protein, PTCH11, Ptch2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Enlarge Image

Western Blot - Anti-Patched / PTCH1 Antibody (A11198)

Western blot analysis of Mouse lung, using Anti-Patched / PTCH1 Antibody (A11198) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Enlarge Image

Immunohistochemistry - Anti-Patched / PTCH1 Antibody (A11198)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Patched / PTCH1 Antibody (A11198) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-Patched / PTCH1 Antibody (A11198)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Patched / PTCH1 Antibody (A11198) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-Patched / PTCH1 Antibody (A11198)

Immunohistochemistry analysis of paraffin-embedded human breast tissue using Anti-Patched / PTCH1 Antibody (A11198) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.