Anti-PRPF31 Antibody (A84273)

Name: Anti-PRPF31 Antibody
Brand: Antibodies.com
SKU: A84273
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PRPF31 for ELISA, WB, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-PRPF31 Antibody

Description

Goat polyclonal antibody to PRPF31.

Applications

ELISA, WB, IF, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human PRPF31.

Sequence

KELGNSLDKCKNNEN

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

60 kDa

Predicted MW

55.4kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DKFZp566J153, hPrp 31, hPrp31, NY BR 99, Pre mRNA processing factor 31, Pre mRNA processing factor 31 homolog, Pre mRNA processing factor 31 homolog (yeast), Pre-mRNA-processing factor 31, Precursor mRNA-processing factor 31, S. cerevisiae, homolog of, Protein 61K, PRP 31, PRP31, PRP31 pre mRNA processing factor 31 homolog, PRP31 pre mRNA processing factor 31 homolog (yeast), PRP31_HUMAN, PRPF 31, RP 11, RP11, Serologically defined breast cancer antigen NY BR 99, Serologically defined breast cancer antigen NY-BR-99, SNRNP61, U4/U6 small nuclear ribonucleoprotein Prp31, U4/U6 snRNP 61 kDa protein

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PRPF31 Antibody (A84273) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in NIH3T3 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PRPF31 Antibody (A84273) at 1µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in HEK293 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PRPF31 Antibody (A84273) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Weak cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-PRPF31 Antibody (A84273)

PRPF31 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PRPF31 Antibody (A84273) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Some cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.