Anti-PRPF3 Antibody (A14795)

Name: Anti-PRPF3 Antibody
Brand: Antibodies.com
SKU: A14795
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PRPF3 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-PRPF3 Antibody

Description

Rabbit polyclonal antibody to PRPF3.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1).

Sequence

MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPTPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hPrp3, HPRP3P, Pre mRNA splicing factor 3, Pre-mRNA-splicing factor 3, Precursor mRNA-processing factor 3, S. crevisiae, homolog of, PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae), Prp3p, PRPF3_HUMAN, RP18, SNRNP90, U4/U6 small nuclear ribonucleoprotein PRP3, U4/U6 snRNP 90 kDa protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PRPF3 Antibody (A14795)

Western blot analysis of THP-1, using Anti-PRPF3 Antibody (A14795) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-PRPF3 Antibody (A14795)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PRPF3 Antibody (A14795) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PRPF3 Antibody (A14795)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-PRPF3 Antibody (A14795) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PRPF3 Antibody (A14795)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PRPF3 Antibody (A14795) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.