Principle of Assay
The BCA method is suitable for the detection of protein concentrations in the range of 20-2,000 µg/mL, the minimum detected protein amount is 0.4 µg, and the sample volume to be tested is 1-20 µL. Unaffected by most ionic and nonionic detergents. Can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween-20, 60, 80 in the sample. However, the BCA method is affected by chelating agents and slightly higher concentrations of reducing agents. It is necessary to ensure that EDTA is less than 10 mM, no EGTA, dithiothreitol (DTT) is less than 1 mM, and ß-mercaptoethanol (ß-Mercaptoethanol) is less than 0.01%. Good linearity with a linear working range from 20 to 2000 µg/mL. Excellent uniformity, exhibits less protein-to-protein variation than dye-binding methods.
