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Anti-Prostate Specific Antigen Antibody (A13836)

Western Blot - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.com Immunohistochemistry - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.com Immunofluorescence - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.com Western Blot - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Prostate Specific Antigen for WB, IHC, ICC/IF and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Prostate Specific Antigen Antibody
Description
Rabbit polyclonal antibody to Prostate Specific Antigen.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-261 of human KLK3 (NP_001639.1).
Sequence
ECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
antigen, prostate-specific, APS, Gamma seminoprotein, Gamma-seminoprotein, hK3, Kallikrein 3, Kallikrein related peptidase 3, Kallikrein-3, KLK 3, KLK2A1, Klk3, KLK3_HUMAN, P-30 antigen, P30 antigen, Prostate-specific antigen, Psa, Semenogelase, Seminin
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Prostate Specific Antigen Antibody (A13836)
Western blot analysis of extracts of LNCaP cells, using Anti-Prostate Specific Antigen Antibody (A13836) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Prostate Specific Antigen Antibody (A13836)
Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Anti-Prostate Specific Antigen Antibody (A13836) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Prostate Specific Antigen Antibody (A13836)
Immunofluorescence analysis of LnCap cells using Anti-Prostate Specific Antigen Antibody (A13836) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Prostate Specific Antigen Antibody (A13836) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-Prostate Specific Antigen Antibody (A13836)
Immunoprecipitation analysis of 300µg extracts of LNCaP cells using 3µg of Anti-Prostate Specific Antigen Antibody (A13836). This Western blot was performed on the immunoprecipitate using Anti-Prostate Specific Antigen Antibody (A13836) at a dilution of 1:2000.
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