Progesterone Receptor (phospho Ser190) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Progesterone Receptor (phospho Ser190) Cell Based ELISA Kit allows for the detection of Progesterone Receptor (phospho Ser190) and the effects that certain stimulation conditions have on Progesterone Receptor (phospho Ser190) expression in different cell lines. Qualitative determination of Progesterone Receptor (phospho Ser190) concentration is achieved by an indirect ELISA format. In essence, the Progesterone Receptor (phospho Ser190) is captured by Anti-Progesterone Receptor (phospho Ser190) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Progesterone Receptor (phospho Ser190) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Progesterone Receptor Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, PRGR_HUMAN, Progestin receptor form A, Progestin receptor form B
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 4: Western Blot - Progesterone Receptor (phospho Ser190) Cell Based ELISA Kit (A102749)
The mouse monoclonal antibody to GAPDH is used as a positive control inProgesterone Receptor (phospho Ser190) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using Progesterone Receptor (phospho Ser190) Cell Based ELISA Kit (A102749)? Please let us know so that we can list the citation on this page.
Alternative products to Progesterone Receptor (phospho Ser190) Cell Based ELISA Kit (A102749)