Figure 1: Western Blot - Anti-Progesterone Receptor Antibody [ARC51400] (A305255)
Western blot analysis of extracts of BT-474 cells, using Anti-Progesterone Receptor Antibody [ARC51400] (A305255) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Progesterone Receptor Antibody [ARC51400] (A305255) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human cervix using Anti-Progesterone Receptor Antibody [ARC51400] (A305255) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human triple negative breast cancer (negative control sample) using Anti-Progesterone Receptor Antibody [ARC51400] (A305255) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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