Immunohistochemistry (IHC) is a technique that uses antibodies for microscopy-based detection of analytes in solid tissue sections. A main advantage of IHC over methods such as western blot and enzyme-linked immunosorbent assay (ELISA) is that it provides information about target localization and abundance relative to other proteins, which can be especially helpful when comparing normal and diseased tissue samples. IHC can be configured for either direct or indirect detection, and typically produces either a chromogenic or fluorescent readout depending on the types of antibody labels that are used. In addition, various approaches have been developed for IHC to provide further signal amplification. Chromogenic detection for IHC involves pairing antibodies labeled with horseradish peroxidase (HRP) or alkaline phosphatase (AP) with an appropriate substrate to generate a colored precipitate that can be visualized with a standard microscope. Commonly used substrates for HRP include 3,3'-diaminobenzidine (DAB), aminoethyl carbazole (AEC), and 3,3’-5-5’-tetramethylbenzidine (TMB), which are respectively catalyzed to produce brown, red, and blue staining. AP substrates include 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT) and BCIP/tetra-NBT (BCIP/TNBT), which give rise to blue and purple coloration. Fluorescent detection instead uses fluorophore-labeled antibodies and requires a fluorescence microscope for sample visualization. While IHC experiments can be based on direct detection using labeled primary antibodies, it is common for indirect detection to be performed, followed by additional signal amplification. One of the best-known strategies is the avidin-biotin-complex (ABC) method, which involves incubating the sample with an unlabeled primary antibody and a biotinylated secondary antibody, before adding large avidin-biotin-enzyme (HRP or AP) complexes to increase the concentration of enzyme at the antigen site. Another approach, known as the labeled-streptavidin biotin (LSAB) method, uses a streptavidin-enzyme conjugate to detect the biotinylated antibody. The LSAB method is considered to provide better tissue penetration than the ABC method due to the smaller size of the detection moiety. When using enzyme- or biotin-labeled secondary antibodies for IHC, it is important to block endogenous peroxidase, phosphatase, or biotin. Endogenous peroxidase activity can be inhibited by incubating the tissue sections in a dilute solution of hydrogen peroxide, while endogenous phosphatase activity is usually blocked with levamisole or dilute acetic acid depending on the tissue source. To block endogenous biotin, it is recommended to incubate the samples in a dilute solution of avidin followed by incubation with biotin (to block any remaining open sites on the avidin molecule). The effectiveness of these treatments is easily confirmed by incubating a spare sample in the relevant substrate.
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A17345) | |
100µl/$95 | |
Description: | Goat anti-rabbit IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ELISA |
Reactivity: | Rabbit |
Conjugate: | HRP |
Goat Anti-Rabbit IgG H&L Antibody (HRP), Cross-Adsorbed (A294888) | |
500µg/$225 | |
Description: | Goat anti-rabbit IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rabbit |
Conjugate: | HRP |
Donkey Anti-Goat IgG H&L Antibody (HRP) (A17357) | |
100µl/$105 | |
Description: | Donkey anti-goat IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC |
Reactivity: | Goat |
Conjugate: | HRP |
Rabbit Anti-Mouse IgG H&L Antibody (HRP) (A301453) | |
1mg/$2251 Citation | |
Description: | Rabbit anti-mouse IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | HRP |
Goat Anti-Mouse IgG H&L Antibody (HRP) (A301445) | |
1mg/$225 | |
Description: | Goat anti-mouse IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | HRP |
Goat Anti-Mouse IgG H&L Antibody (FITC), Cross-Adsorbed (A301669) | |
500µg/$225 | |
Description: | Goat anti-mouse IgG H&L (FITC) secondary antibody. |
Applications: | IHC, ICC, Flow Cytometry, IF |
Reactivity: | Mouse |
Conjugate: | FITC |
Goat Anti-Chicken IgM Antibody (A282909) | |
1mg/$270 | |
Description: | Goat anti-chicken IgM secondary antibody. |
Applications: | WB, ELISA, IHC-Fr, Immunodiffusion, ICC |
Reactivity: | Chicken |
Conjugate: | Unconjugated |
Goat Anti-Rabbit IgG H&L Antibody (Cyanine 5), Cross-Adsorbed (A294878) | |
500µg/$250 | |
Description: | Goat anti-rabbit IgG H&L (Cyanine 5) secondary antibody. |
Applications: | WB, IHC, ICC, Flow Cytometry, IF |
Reactivity: | Rabbit |
Conjugate: | Cyanine 5 |
Rabbit F(ab')2 Anti-Mouse IgG Antibody (HRP), Cross-Absorbed (A282601) | |
1mg/$670 | |
Description: | Rabbit F(ab')2 anti-mouse IgG (HRP) secondary antibody. |
Applications: | WB, IHC-P, IHC-Fr, ELISA, IHC-R |
Reactivity: | Mouse, Rat |
Conjugate: | HRP |
Goat Anti-Monkey IgG H&L Antibody (FITC) (A282431) | |
1mg/$300 | |
Description: | Goat anti-monkey IgG H&L (FITC) secondary antibody. |
Applications: | IHC-Fr, IF, Flow Cytometry, ICC |
Reactivity: | Monkey, Human, African green monkey , Cynomolgus monkey, Baboon, Chimpanzee, Rhesus Monkey |
Conjugate: | FITC |
Goat Anti-Rat IgG1 + IgG2a + IgG2b + IgG2c + IgA + IgM H&L Antibody (HRP) (A116278) | |
1ml/$610 | |
Description: | Goat anti-Rat IgG1 + IgG2a + IgG2b + IgG2c + IgA + IgM H&L (HRP) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Rat |
Conjugate: | HRP |
Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM223] antibody to Mouse IgG2c (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Mouse IgG Fc Antibody (HRP) (A301474) | |
1mg/$275 | |
Description: | Goat anti-mouse IgG Fc (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | HRP |
Anti-Mouse IgA Antibody [RM220] (Biotin) (A121268) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM220] antibody to Mouse Mouse IgA (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG Antibody [RM104] (Biotin) (A121283) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM104] antibody to Mouse IgG (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Rabbit Anti-Goat IgG H&L Antibody (HRP) (A17358) | |
100µl/$95 | |
Description: | Rabbit anti-goat IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC |
Reactivity: | Goat |
Conjugate: | HRP |
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A294801) | |
1mg/$225 | |
Description: | Goat anti-rabbit IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rabbit |
Conjugate: | HRP |
Anti-Mouse Ig Kappa Light Chain Antibody [RM103] (Biotin) (A121252) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM103] antibody to Mouse Mouse Ig Kappa Light Chain (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Rabbit IgG H&L Antibody (FITC), Cross-Adsorbed (A294887) | |
500µg/$225 | |
Description: | Goat anti-rabbit IgG H&L (FITC) secondary antibody. |
Applications: | IHC, ICC, Flow Cytometry, IF |
Reactivity: | Rabbit |
Conjugate: | FITC |
Goat Anti-Mouse IgG H&L Antibody (AP) (A301438) | |
1mg/$235 | |
Description: | Goat anti-mouse IgG H&L (AP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | AP |
Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM107] antibody to Mouse IgG2a Kappa (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Mouse IgG + IgM + IgA H&L Antibody (Biotin) (A301526) | |
500µg/$260 | |
Description: | Goat anti-mouse IgG + IgM + IgA H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin) (A121274) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG02] antibody to Rabbit IgG Fc (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG01] antibody to Rabbit IgG F(ab) (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A282589) | |
1mg/$490 | |
Description: | Goat anti-rabbit IgG H&L (HRP) secondary antibody. |
Applications: | WB, ELISA, IHC-Fr |
Reactivity: | Rabbit |
Conjugate: | HRP |
Goat Anti-Rabbit IgG H&L Antibody (Cyanine 3), Cross-Adsorbed (A294877) | |
500µg/$250 | |
Description: | Goat anti-rabbit IgG H&L (Cyanine 3) secondary antibody. |
Applications: | IHC, ICC, Flow Cytometry, IF |
Reactivity: | Rabbit |
Conjugate: | Cyanine 3 |
Goat Anti-Rabbit IgG H&L Antibody (FITC), Cross-Adsorbed (A294948) | |
1mg/$250 | |
Description: | Goat anti-rabbit IgG H&L (FITC) secondary antibody. |
Applications: | IHC, ICC, Flow Cytometry, IF |
Reactivity: | Rabbit |
Conjugate: | FITC |
Goat Anti-Rabbit IgG Fc Antibody (HRP) (A294847) | |
1mg/$275 | |
Description: | Goat anti-rabbit IgG Fc (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rabbit |
Conjugate: | HRP |
Anti-Mouse IgG2a Antibody [RM219] (Biotin) (A121263) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM219] antibody to Mouse IgG2a (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Rabbit Anti-Sheep IgG H&L Antibody (HRP) (A17336) | |
100µl/$105 | |
Description: | Rabbit anti-sheep IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC |
Reactivity: | Sheep |
Conjugate: | HRP |
Anti-Mouse IgG3 Antibody [RM218] (Biotin) (A121265) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM218] antibody to Mouse IgG3 (Biotin). |
Applications: | WB, IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Dog IgE Antibody (HRP) (A282567) | |
500µg/$535 | |
Description: | Goat anti-dog IgE (HRP) secondary antibody. |
Applications: | WB, ELISA, IHC-Fr |
Reactivity: | Canine |
Conjugate: | HRP |
Goat Anti-Rabbit IgG H&L Antibody (TRITC) (A17346) | |
100µl/$105 | |
Description: | Goat anti-rabbit IgG H&L (TRITC) secondary antibody. |
Applications: | IHC, ICC/IF, Flow Cytometry |
Reactivity: | Rabbit |
Conjugate: | TRITC |
Goat Anti-Guinea Pig IgG H&L Antibody (HRP) (A17347) | |
100µl/$95 | |
Description: | Goat anti-guinea pig IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC |
Reactivity: | Guinea Pig |
Conjugate: | HRP |
Anti-Mouse IgG Fc Antibody [RMG06] (Biotin) (A121258) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG06] antibody to Mouse IgG Fc (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG F(ab) Antibody [RMG05] (Biotin) (A121260) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG05] antibody to Mouse IgG F(ab) (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Rat IgG H&L + IgM H&L Antibody, Cross-Absorbed (A284113) | |
1mg/$450 | |
Description: | Goat anti-rat IgG H&L + IgM H&L secondary antibody. |
Applications: | WB, IHC-P, ELISA, IHC-Fr, Flow Cytometry, ICC, FLISA |
Reactivity: | Rat |
Conjugate: | Unconjugated |
Anti-Mouse IgG Antibody [RMG07] (Biotin) (A121261) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG07] antibody to Mouse IgG (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Donkey Anti-Rabbit IgG H&L Antibody (HRP) (A294819) | |
1mg/$225 | |
Description: | Donkey anti-rabbit IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rabbit |
Conjugate: | HRP |
Rabbit Anti-Guinea Pig IgG H&L Antibody (Biotin) (A300759) | |
1mg/$200 | |
Description: | Rabbit anti-guinea pig IgG H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Guinea Pig |
Conjugate: | Biotin |
Goat Anti-Human IgG H&L Antibody (HRP) (A17337) | |
100µl/$95 | |
Description: | Goat anti-human IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC |
Reactivity: | Human |
Conjugate: | HRP |
Donkey Anti-Sheep IgG H&L Antibody (HRP) (A294959) | |
1mg/$225 | |
Description: | Donkey anti-sheep IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Sheep |
Conjugate: | HRP |
Anti-Mouse IgM Antibody [RM109] (Biotin) (A121267) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM109] antibody to Mouse IgM (Biotin). |
Applications: | ICC, IHC, IP, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Sheep Anti-Rabbit IgG H&L Antibody (FITC) (A294790) | |
1mg/$200 | |
Description: | Sheep anti-rabbit IgG H&L (FITC) secondary antibody. |
Applications: | IHC, ICC, Flow Cytometry, IF |
Reactivity: | Rabbit |
Conjugate: | FITC |
Goat Anti-Dog IgA Antibody (A282912) | |
1mg/$260 | |
Description: | Goat anti-dog IgA secondary antibody. |
Applications: | WB, ELISA, IHC-Fr, Immunodiffusion |
Reactivity: | Canine, Mustelid, Ferret, Wolf |
Conjugate: | Unconjugated |
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300686) | |
1mg/$225 | |
Description: | Donkey anti-goat IgG H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Goat |
Conjugate: | HRP |
Goat Anti-Mouse IgG + IgM + IgA H&L Antibody (HRP) (A301528) | |
500µg/$275 | |
Description: | Goat anti-mouse IgG + IgM + IgA H&L (HRP) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | HRP |
Goat Anti-Mouse Kappa Light Chain Antibody (FITC) (A301456) | |
1mg/$375 | |
Description: | Goat anti-mouse kappa light chain (FITC) secondary antibody. |
Applications: | IHC, ICC, IF |
Reactivity: | Mouse |
Conjugate: | FITC |
Mouse Anti-Human IgA2 Antibody (HRP) (A116348) | |
200µg/$845 | |
Description: | Mouse anti-Human IgA2 (HRP) secondary antibody. |
Applications: | ELISA, Dot, WB, ICC, IHC-Fr, IHC-P |
Reactivity: | Human |
Conjugate: | HRP |
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