Biotin is a stable small molecule known for its high-affinity, non-covalent interaction with Streptavidin / Avidin. The strength and specificity of this bond underpins the versatile roles of biotin-conjugated secondary antibodies in a variety of immunoassays and protein enrichment protocols; including: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Enzyme-Linked Immunosorbent Assays (ELISA), Western Blotting (WB), Flow Cytometry, Affinity Purification and Immunoprecipitation. The multivalent properties of streptavidin confer high sensitivity to biotin conjugation systems enabling them to robustly detect low abundance targets. Signal amplification is achieved through the presence of multiple (>4) biotin molecules on each antibody, which is compounded by the tetravalent binding mode of streptavidin molecules. In the context of affinity purification and immunoprecipitation experiments, this facilitates efficient and specific target isolation by affording high stringency wash conditions. Target visualisation using biotin-streptavidin systems requires streptavidin conjugation to fluorescent dyes or reporter enzymes such as HRP or AP. For the latter, additional signal amplification is achieved through catalysed conversion of chromogenic, fluorescent or chemiluminescent substrates. Explore our portfolio of full length and fragmented biotin-conjugated secondary antibodies below, that are raised against a variety of primary antibody host species and their isotypes. Many of our biotinylated secondary antibodies are also available cross-adsorbed - to limit cross-species reactivity and are highly specific as a result. Note: The use of biotin-conjugates in target visualisation involves complex protocols incurring higher costs. While this may be necessary to detect very low abundance proteins, researchers should consider whether sufficiently robust signal could be achieved using HRP labeled secondary antibodies, AP labeled secondary antibodies or secondary antibodies conjugated to fluorescent dyes.
Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM223] antibody to Mouse IgG2c (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgA Antibody [RM220] (Biotin) (A121268) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM220] antibody to Mouse Mouse IgA (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG Antibody [RM104] (Biotin) (A121283) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM104] antibody to Mouse IgG (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM107] antibody to Mouse IgG2a Kappa (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Mouse IgG + IgM + IgA H&L Antibody (Biotin) (A301526) | |
500µg/$260 | |
Description: | Goat anti-mouse IgG + IgM + IgA H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin) (A121274) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG02] antibody to Rabbit IgG Fc (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG01] antibody to Rabbit IgG F(ab) (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Anti-Mouse Ig Kappa Light Chain Antibody [RM103] (Biotin) (A121252) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM103] antibody to Mouse Mouse Ig Kappa Light Chain (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG2a Antibody [RM219] (Biotin) (A121263) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM219] antibody to Mouse IgG2a (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced) |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG3 Antibody [RM218] (Biotin) (A121265) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM218] antibody to Mouse IgG3 (Biotin). |
Applications: | WB, IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG Antibody [RMG07] (Biotin) (A121261) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG07] antibody to Mouse IgG (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Rabbit Anti-Guinea Pig IgG H&L Antibody (Biotin) (A300759) | |
1mg/$200 | |
Description: | Rabbit anti-guinea pig IgG H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Guinea Pig |
Conjugate: | Biotin |
Anti-Mouse IgM Antibody [RM109] (Biotin) (A121267) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM109] antibody to Mouse IgM (Biotin). |
Applications: | ICC, IHC, IP, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG Fc Antibody [RMG06] (Biotin) (A121258) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG06] antibody to Mouse IgG Fc (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG F(ab) Antibody [RMG05] (Biotin) (A121260) | |
50µg/$545 | |
Description: | Recombinant goat monoclonal [RMG05] antibody to Mouse IgG F(ab) (Biotin). |
Applications: | IP, ICC, IHC, Flow Cytometry, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Human IgA Fc Antibody (Biotin) (A115919) | |
1ml/$610 | |
Description: | Goat anti-Human IgA Fc (Biotin) secondary antibody. |
Applications: | ELISA, WB, Dot, ICC, IHC, IHC-P |
Reactivity: | Human |
Conjugate: | Biotin |
Rabbit Anti-Rat IgG1 + IgG2a + IgG2b + IgG2c + IgA + IgM H&L Antibody (Biotin) (A116074) | |
1ml/$735 | |
Description: | Rabbit anti-Rat IgG1 + IgG2a + IgG2b + IgG2c + IgA + IgM H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Rat |
Conjugate: | Biotin |
Goat Anti-Chicken IgM Fc Antibody (Biotin) (A115783) | |
1ml/$610 | |
Description: | Goat anti-Chicken IgM Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Chicken |
Conjugate: | Biotin |
Rabbit Anti-Mouse IgG H&L Antibody (Biotin) (A301451) | |
1mg/$200 | |
Description: | Rabbit anti-mouse IgG H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Mouse |
Conjugate: | Biotin |
Anti-Mouse IgG1 Antibody [RM106] (Biotin) (A121284) | |
50µg/$495 | |
Description: | Recombinant rabbit monoclonal [RM106] antibody to Mouse IgG1 (Biotin). |
Applications: | ELISA, WB, IHC |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Human IgG Fc Antibody (Biotin), Cross-Adsorbed (A301290) | |
500µg/$275 | |
Description: | Goat anti-human IgG Fc (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Human |
Conjugate: | Biotin |
Goat Anti-Rat IgG H&L Antibody (Biotin) (A294630) | |
1mg/$200 | |
Description: | Goat anti-rat IgG H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rat |
Conjugate: | Biotin |
Goat Anti-Rabbit IgG H&L Antibody (Biotin) (A294795) | |
1mg/$200 | |
Description: | Goat anti-rabbit IgG H&L (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Goat Anti-Guinea Pig IgG2 Antibody (Biotin) (A115716) | |
1ml/$735 | |
Description: | Goat anti-Guinea Pig IgG2 (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Guinea Pig |
Conjugate: | Biotin |
Goat Anti-Human IgG F(ab) Antibody (Biotin) (A115887) | |
1ml/$490 | |
Description: | Goat anti-Human IgG F(ab) (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Goat Anti-Mouse IgG2 Antibody (Biotin) (A116033) | |
1ml/$610 | |
Description: | Goat anti-Mouse IgG2 (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Rabbit IgG Fc Antibody (Biotin) (A116169) | |
1ml/$735 | |
Description: | Goat anti-Rabbit IgG Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Goat Anti-Turkey IgG H&L Antibody (Biotin) (A116295) | |
1ml/$490 | |
Description: | Goat anti-Turkey IgG H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Turkey |
Conjugate: | Biotin |
Mouse Anti-Human IgM Antibody (Biotin) (A116411) | |
200µg/$845 | |
Description: | Mouse anti-Human IgM (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Rabbit Anti-Human Kappa Light Chain Antibody (Biotin) (A117051) | |
10mg/$610 | |
Description: | Rabbit anti-Human Kappa Light Chain (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Rabbit Anti-Porcine IgG Fc Antibody (Biotin) (A117266) | |
1ml/$735 | |
Description: | Rabbit anti-Porcine IgG Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Porcine |
Conjugate: | Biotin |
Goat Anti-Human IgG H&L Antibody (Biotin) (A115722) | |
1ml/$490 | |
Description: | Goat anti-Human IgG H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Goat Anti-Human IgG + IgA + IgM Fc Antibody (Biotin) (A115904) | |
1ml/$610 | |
Description: | Goat anti-Human IgG + IgA + IgM Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Goat Anti-Mouse IgG2a Antibody (Biotin) (A116039) | |
1ml/$735 | |
Description: | Goat anti-Mouse IgG2a (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Rabbit IgG H&L Antibody (Biotin) (A116174) | |
1ml/$490 | |
Description: | Goat anti-Rabbit IgG H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Mouse Anti-Human Bence Jones kappa Antibody (Biotin) (A116322) | |
200µg/$610 | |
Description: | Mouse anti-Human Bence Jones kappa (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Mouse Anti-Human Secretory Component Antibody (Biotin) (A116423) | |
200µg/$845 | |
Description: | Mouse anti-Human Secretory Component (Biotin) secondary antibody. |
Applications: | Dot, ELISA, WB, ICC, IHC |
Reactivity: | Human |
Conjugate: | Biotin |
Rabbit Anti-Human Lambda Light Chain Antibody (Biotin) (A117056) | |
10mg/$610 | |
Description: | Rabbit anti-Human Lambda Light Chain (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Rabbit Anti-Porcine IgG H&L Antibody (Biotin) (A117270) | |
1ml/$490 | |
Description: | Rabbit anti-Porcine IgG H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Porcine |
Conjugate: | Biotin |
Goat Anti-Mouse IgG1 + IgG2a + IgG2b + IgG3 + IgA + IgM Fc Antibody (Biotin) (A115731) | |
1ml/$610 | |
Description: | Goat anti-Mouse IgG1 + IgG2a + IgG2b + IgG3 + IgA + IgM Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Human IgG + IgA + IgM H&L Antibody (Biotin) (A115912) | |
1ml/$475 | |
Description: | Goat anti-Human IgG + IgA + IgM H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Goat Anti-Mouse IgG2b Antibody (Biotin) (A116046) | |
1ml/$735 | |
Description: | Goat anti-Mouse IgG2b (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Goat Anti-Rabbit IgM Fc Antibody (Biotin) (A116180) | |
1ml/$735 | |
Description: | Goat anti-Rabbit IgM Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Rabbit |
Conjugate: | Biotin |
Mouse Anti-Human Bence Jones lambda Antibody (Biotin) (A116328) | |
200µg/$610 | |
Description: | Mouse anti-Human Bence Jones lambda (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Human |
Conjugate: | Biotin |
Rabbit Anti-Goat IgG H&L Antibody (Biotin) (A116911) | |
1ml/$735 | |
Description: | Rabbit anti-Goat IgG H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Goat |
Conjugate: | Biotin |
Rabbit Anti-Mouse IgG1 + IgG2a + IgG2b + IgG3 + IgA + IgM H&L Antibody (Biotin) (A117135) | |
1ml/$735 | |
Description: | Rabbit anti-Mouse IgG1 + IgG2a + IgG2b + IgG3 + IgA + IgM H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Mouse |
Conjugate: | Biotin |
Sheep Anti-Guinea Pig IgG Fc Antibody (Biotin) (A117292) | |
1ml/$735 | |
Description: | Sheep anti-Guinea Pig IgG Fc (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Guinea Pig |
Conjugate: | Biotin |
Goat Anti-Rat IgG Fc Antibody (Biotin) (A294671) | |
1mg/$260 | |
Description: | Goat anti-rat IgG Fc (Biotin) secondary antibody. |
Applications: | WB, IHC, ICC, ELISA |
Reactivity: | Rat |
Conjugate: | Biotin |
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A115745) | |
1ml/$490 | |
Description: | Donkey anti-Goat IgG H&L (Biotin) secondary antibody. |
Applications: | ELISA, ICC, IHC-P, Dot, WB |
Reactivity: | Goat |
Conjugate: | Biotin |
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