Influenza B Virus Hemagglutinin Antibodies

Explore our collection of highly validated Influenza B Virus Hemagglutinin antibodies suitable for WB, ICC/IF, ELISA and IP that react with Influenza B Virus samples.

2 Products
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region..
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