Golgi Markers

The Golgi apparatus is located in a peri-nuclear position and receives proteins from the endoplasmic reticulum (ER) then processes and sorts these proteins for transport to their final sub-cellular destinations. In addition, the Golgi serves to synthesise glycolipids and sphingomyelin. Morphologically the Golgi apparatus is composed of flattened membrane-enclosed sacs or compartments known as cisternae as well as many small vesicles. The Golgi apparatus comprises four distinct regions known as the cisGolgi network, the Golgi stack (which comprises the medial and trans sub-compartments) and the transGolgi network. In most vertebrate cells, the Golgi stacks are connected laterally by tubules to form the Golgi ribbon, which is flanked by the cis and trans Golgi networks. Proteins from the ER are transported to the ER-Golgi intermediate compartment (ERGIC, also known as vesicular tubular clusters: VTCs) in coat protein II (COPII) vesicles. The ERGIC is a sub-cellular compartment that is formed from the fusion of many COPII vesicles and is biochemically distinct from the ER and the Golgi apparatus. COPI vesicles mediate retrograde transport of escaped ER residents back to the ER as well as facilitating intra-Golgi transport. From the ERGIC, proteins then enter the Golgi apparatus at the cis Golgi network, from where they move through the medial and trans compartments of the Golgi stack. It is within the Golgi stack that the majority of the metabolic reactions and processes of the Golgi occur. Golgi enzymes are responsible for post-translational modifications such as protein glycosylation, sulfation and phosphorylation. The Golgi-modified proteins subsequently move to the transGolgi network for sorting. Some Golgi-mediated modifications serve as signals to direct proteins to their final cellular destinations including the lysosomes, the plasma membrane or the cell exterior. There are numerous specific Golgi markers. Golgi matrix protein 130 (GM130/Golgin subfamily member A2, GOLGA2) is a specific cisGolgi network marker. Whilst, trans-Golgi network protein 2 (TGN46) and golgin A1/97 (GOLGA1) serve as transGolgi network markers. We offer a large range of Golgi marker antibodies including RCAS1 antibodies, GM130 antibodies, TGN46 antibodies, COG2 antibodies, and GLG1 antibodies. Our antibodies are available in many different host species, formulations, and conjugates, and they have been validated in multiple research applications, and with samples derived from multiple species.