Enzyme-linked immunosorbent assays (ELISAs) are used for detecting analytes of interest in solution, typically in 96-well or 384-well microplate formats. They are one of the most popular types of immunoassays due to their high specificity and sensitivity, ease of use, and the fact that they are able to provide quantitative data. ELISAs can be used with a broad range of samples, including cell lysates, culture supernatants, and tissue homogenates, as well as serum, urine, and saliva, and have applicability for basic research through to clinical testing. ELISAs are classified into four main groups (direct, indirect, sandwich, and competitive) depending on how they are configured. In a direct ELISA, the antigen (along with any other proteins in the sample) is bound to the microplate wells and detected with a labeled primary antibody. An indirect ELISA is similar, but includes an extra protocol step since a labeled secondary antibody is used for detection. Sandwich ELISAs typically use matched pairs of antibodies for analyte capture and detection and are the most popular ELISA format due to their superior specificity. Direct, indirect, and sandwich ELISA can all be converted into a competitive ELISA, which is distinct from the other ELISA types in that it measures signal interference rather than signal accumulation. Specifically, a competitive ELISA is based on competition between a target analyte and a reference molecule for binding to a relevant partner, which could be either a protein antigen or an antibody depending on how the assay is configured. A common way of setting up a competitive ELISA involves coating the microplate wells with a known amount of a reference antigen before washing and blocking. The sample is then added to the wells along with a labeled detection antibody, which may either bind to the immobilized reference antigen or to the antigen that is free in solution. In this scenario, the more antigen there is in the sample, the less antibody is captured on the microplate wells and the lower the assay signal. Because competitive ELISAs are generally more challenging to develop and optimize than the other ELISA formats, they tend to be reserved for situations where only one antibody is available for the target, or for detecting small antigens that cannot be bound by two different antibodies simultaneously.
FLAG Tag ELISA Kit (A303885) | |
96T/$770 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 ng/ml |
Dopamine ELISA Kit (A75362) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.563-100 ng/ml |
Pregnenolone ELISA Kit (A73791) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 pg/ml |
Hyaluronic Acid ELISA Kit (A75464) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.563-100 ng/ml |
Mouse Glucagon ELISA Kit (A80003) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 31.25-2000 pg/ml |
Bovine Aprotinin ELISA Kit (A5146) | |
48T - 96T/$650 – $835 | |
Applications: | Competitive ELISA |
Reactivity: | Bovine |
Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Range: | 1.56-100 ng/ml |
Human Sphingosine-1-phosphate ELISA Kit (A73746) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 3.125-200 ng/ml |
Mouse Heparan Sulfate ELISA Kit (A73809) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 31.25-2000 ng/ml |
Mouse 4-Hydroxynonenal ELISA Kit (A76021) | |
96T/$6251 Citation | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 31.25-2000 pg/ml |
5-Hydroxytryptamine ELISA Kit (A1826) | |
48T - 96T/$570 – $735 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma or other biological fluids. |
Range: | 15.6-1000 ng/ml |
Human Angiotensin 1-7 ELISA Kit (A6594) | |
48T - 96T/$610 – $770 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Range: | 7.81-500 pg/ml |
Mouse Estrogen ELISA Kit (A74581) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 pg/ml |
Human Cholecystokinin ELISA Kit (A79184) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 pg/ml |
4-Hydroxynonenal ELISA Kit (A86962) | |
96T/$625 | |
Applications: | Competitive ELISA |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 31.25-2000 pg/ml |
Sensitivity: | 18.75 pg/ml |
Human Malondialdehyde ELISA Kit (A79849) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 7.813-500 ng/ml |
Chicken Triiodothyronine/T3 ELISA Kit (A246875) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Chicken |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.156-10 ng/ml |
Corticosterone ELISA Kit (A74369) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 2.813-180 ng/ml |
25-Hydroxy Vitamin D3 ELISA Kit (A303874) | |
96T/$770 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 3.125-200 ng/ml |
Mouse ACTH ELISA Kit (A77635) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 12.5-800 pg/ml |
3-Methoxy-4-hydroxyphenylglycol ELISA Kit (A247120) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.156-10 ng/ml |
Cortisol ELISA Kit (A73905) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.391-25 ng/ml |
Malondialdehyde ELISA Kit (A74477) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 7.813-500 ng/ml |
Sheep Prolactin/PRL ELISA Kit (A87526) | |
96T/$730 | |
Applications: | Competitive ELISA |
Reactivity: | Sheep |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.313-20 ng/ml |
Human Oxytocin ELISA Kit (A79596) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 pg/ml |
Oxytocin ELISA Kit (A74919) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 pg/ml |
Vasopressin ELISA Kit (A86970) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.313-20 pg/ml |
Bovine IgA ELISA Kit (A79011) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Bovine |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 3.125-200 ng/ml |
Human Neopterin ELISA Kit (A74097) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.156-10 ng/ml |
Human GABA ELISA Kit (A76581) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 31.25-2000 pg/ml |
Protectin D1 ELISA Kit (A87006) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.156-10 ng/ml |
Noradrenaline/Norepinephrine ELISA Kit (A74229) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 15.625-1000 pg/ml |
Rat Endothelin 1 ELISA Kit (A79981) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Rat |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.25-80 pg/ml |
Hyaluronic Acid ELISA Kit (A2118) | |
48T - 96T/$570 – $735 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma or other biological fluids. |
Range: | 6.25-400 ng/ml |
Ceramide ELISA Kit (A326471) | |
96T/$640 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. |
Range: | 31.2-2000 pg/ml |
Human Angiotensin II ELISA Kit (A74653) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 31.25-2000 pg/ml |
Human Sphingomyelin ELISA Kit (A75019) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 3.125-200 ng/ml |
Rat Estradiol ELISA Kit (A80286) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Rat |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 12.5-800 pg/ml |
Human Melatonin ELISA Kit (A79861) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 7.813-500 pg/ml |
Endothelin-1 ELISA Kit (A247159) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.25-80 pg/ml |
Mouse Testosterone ELISA Kit (A73972) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples. |
Range: | 31.25-2000 pg/ml |
Androstenedione ELISA Kit (A3192) | |
48T - 96T/$570 – $735 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Range: | 0.312-20 ng/ml |
Human Ghrelin ELISA Kit (A78157) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.875-120 pg/ml |
Rabbit Estradiol ELISA Kit (A303857) | |
96T/$970 | |
Applications: | Competitive ELISA |
Reactivity: | Rabbit |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 12.5-800 pg/ml |
Human Angiotensin II ELISA Kit (A1645) | |
48T - 96T/$530 – $670 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma or other biological fluids. |
Range: | 3.12-200 pg/ml |
Vitamin B12 ELISA Kit (A3003) | |
48T - 96T/$570 – $735 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates or other biological fluids. |
Range: | 4.68-300 pg/ml |
Human Serum Indoxyl Sulfate ELISA Kit (A86937) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.563-100 ng/ml |
Bovine Serum Albumin ELISA Kit (A247091) | |
96T/$670 | |
Applications: | Competitive ELISA |
Reactivity: | Bovine |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.156-10 µg/ml |
Human Endothelin 1 ELISA Kit (A78052) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Human |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 1.25-80 pg/ml |
Mouse FSH-R ELISA Kit (A78102) | |
96T/$570 | |
Applications: | Competitive ELISA |
Reactivity: | Mouse |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 2.344-150 mIU/ml |
LPS ELISA Kit (A247372) | |
96T/$625 | |
Applications: | Competitive ELISA |
Reactivity: | Universal |
Sample Type: | Serum, plasma, tissue homogenates, and other biological fluids. |
Range: | 0.313-20 µg/ml |
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