Epitope Tag Antibodies

His Tag

His tag, also known as the polyhistidine tag, is an artificial peptide consisting of six to nine histidine residues.

FLAG Tag

The FLAG tag, otherwise known as the DYKDDDDK tag, is an artificial octapeptide that is not derived from a naturally occurring protein. This tag contains a recognition sequence for the protease Enterokinase, enabling cleavage of FLAG-tagged proteins and thus tag removal.

Myc Tag

The Myc epitope tag is derived from the C-terminal, amino acids 410-419, of the c-Myc protein, which is a transcription factor involved in cell division.

T7 Tag

The T7 epitope tag is a peptide of 11 amino acids encoded in the leader sequence of the T7 bacteriophage gene 10, which encodes a T7 major capsid protein whose exact function remains to be elucidated.

V5 Tag

The V5 epitope tag is derived from an epitope (either 14 or 9 amino acids) present on the P and V proteins of the paramyxovirus of the Simian Virus 5 family.

Fluorescent Protein Tags

Fluorescent protein tags represent another useful tool that can be used in a variety of laboratory applications including cell tracking, sub-cellular localisation and cell sorting. Commonly used fluorescent protein tags include Green Fluorescent Protein (GFP) - derived from the jellyfish Aequorea victoria - and Red Fluorescent Protein (RFP). RFP is a variant of DsRed - originally isolated from the sea anemone, Discosoma sp, based on its homology to jellyfish GFP – and other common variants include mCherry, mPlum, and tdTomato. We offer a comphrensive range of fluorescent protein tag antibodies, including antibodies against DENDRA2, FITC, GFP, HRP, mCherry, mPlum, RFP, and tdTomato.

Figure 3: Immunofluorescent analysis of transfected HEK293 cells with a mCherry-HA construct, in red, and stained with Anti-mCherry Antibody (A85306), dilution 1:1,000, in green. The blue is Hoechst staining of nuclear DNA. Anti-mCherry Antibody (A85306) reveals mCherry protein expressed only in transfected cells which appear golden in color.

Figure 4: Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with Anti-GFP Antibody (A85298), dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Anti-GFP Antibody (A85298) reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.