Figure 1: Western Blot - Anti-Presenilin 1 Antibody (A304926)
Western blot analysis of mouse Kidney, liver showing detection of ~52.7 kDa Presenilin 1 protein using Anti-Presenilin 1 Antibody (A304926) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse Liver. Lane 3: mouse Kidney. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 42.7 kDa band is isoform 5.
Immunohistochemistry analysis of human liver, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Presenilin 1 Antibody (A304926) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X.
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