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Anti-PRAF1 Antibody (A90543)

Western Blot - Anti-PRAF1 Antibody (A90543) - Antibodies.com Western Blot - Anti-PRAF1 Antibody (A90543) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to PRAF1 for WB and IP.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-PRAF1 Antibody
Description
Rabbit polyclonal antibody to PRAF1.
Applications
WB, IP
Dilutions
WB: 1:500-1:2,000, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
Sequence
MAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIEAFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDFQIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRRVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
53 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PRAF1 Antibody (A90543) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PRAF1 Antibody (A90543)
Western blot analysis of extracts of various cell lines, using Anti-PRAF1 Antibody (A90543) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-PRAF1 Antibody (A90543) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-PRAF1 Antibody (A90543)
Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-PRAF1 Antibody (A90543). This Western blot was performed on the immunoprecipitate using Anti-PRAF1 Antibody (A90543) at a dilution of 1:1000.
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