Mouse monoclonal [PCRP-PPARD-4C2] antibody to PPAR delta.
Specificity
Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARa, PPARb (also designated PPAR) and PPARg, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARa is abundant in primary hepatocytes, where it regulates the expression of proteins involved in fatty acid metabolism. PPARb is the most widely distributed subtype and is often expressed at high levels. PPARg is predominantly seen in adipose tissue, where it plays a critical role in regulating adipocyte differentiation. Interestingly, both the orphan nuclear hormone receptor LXRa and thyroid receptor (TR) have been shown to act as antagonists of PPARa/ RXRa binding to PPREs.
Applications
Flow Cytometry
Positive Control
HeLa or MCF-7 cells.
Dilutions
Flow Cytometry: 1-2 µg/million cells
Reactivity
Human
Cross Reactivity
This antibody is predicted to cross react with Chicken.
Immunogen
Recombinant fragment (around Amino Acids 72-146) of human PPARD protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-PPARD-4C2
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
49.9 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
NR1C2, NUC1, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, Peroxisome proliferator-activated receptor delta, PPAR-beta, PPAR-delta, PPARB, PPARD
