Anti-PP2A-alpha Antibody (A15336)

Name: Anti-PP2A-alpha Antibody
Brand: Antibodies.com
SKU: A15336
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PP2A-alpha for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PP2A-alpha Antibody

Description

Rabbit polyclonal antibody to PP2A-alpha.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PP2A Catalytic a (NP_002706.1).

Sequence

MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

PP2A alpha, PP2Aalpha, PP2AA_HUMAN, PP2Ac, PP2CA, PP2Calpha, PPP2CA, Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform, Protein phosphatase 2 catalytic subunit alpha isoform, Protein phosphatase 2, catalytic subunit, alpha isozyme, Protein phosphatase 2A catalytic subunit, alpha isoform, Replication protein C, RP C, RP-C, RPC, Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PP2A-alpha Antibody (A15336)

Western blot analysis of various lysates, using Anti-PP2A-alpha Antibody (A15336) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-PP2A-alpha Antibody (A15336)

Immunofluorescence analysis of HeLa cells using Anti-PP2A-alpha Antibody (A15336) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-PP2A-alpha Antibody (A15336)

Immunofluorescence analysis of NIH/3T3 cells using Anti-PP2A-alpha Antibody (A15336) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-PP2A-alpha Antibody (A15336)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-PP2A-alpha Antibody (A15336). This Western blot was performed on the immunoprecipitate using Anti-PP2A-alpha Antibody (A15336) at a dilution of 1:1000.