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Anti-POLRMT Antibody (A88198)

Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.com Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.com Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.com Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to POLRMT for WB, ICC/IF and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-POLRMT Antibody
Description
Rabbit polyclonal antibody to POLRMT.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:50-1:100
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
Sequence
SSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
139 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
APOLMT, DNA-directed RNA polymerase, DNA-directed RNA polymerase mitochondrial, h-mtRPOL, mitochondrial, mtRNA polymerase, MTRNAP, MtRPOL, polymerase (RNA) mitochondrial (DNA directed), polymerase, RNA, mitochondrial, RPOM, RPOM_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-POLRMT Antibody (A88198)
Western blot analysis of extracts of various cell lines, using Anti-POLRMT Antibody (A88198) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-POLRMT Antibody (A88198)
Immunofluorescence analysis of C6 cells using Anti-POLRMT Antibody (A88198) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-POLRMT Antibody (A88198)
Immunofluorescence analysis of U-2 OS cells using Anti-POLRMT Antibody (A88198) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-POLRMT Antibody (A88198)
Immunoprecipitation analysis of 200µg extracts of HepG2 cells using 3µg of Anti-POLRMT Antibody (A88198). This Western blot was performed on the immunoprecipitate using Anti-POLRMT Antibody (A88198) at a dilution of 1:1000.
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