Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, DNA-directed RNA polymerases I, II, and III subunit RPABC3, hRPB8, RNA polymerases I, II, and III subunit ABC3, RPB17, RPB8 homolog
Western blot analysis of extracts of various cell lines, using Anti-POLR2H Antibody (A88536) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation analysis of 200µg extracts of MCF7 cells using 1µg of Anti-POLR2H Antibody (A88536). This Western blot was performed on the immunoprecipitate using Anti-POLR2H Antibody (A88536) at a dilution of 1:1000.
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