Anti-POLR2A (phospho Ser1619) Antibody (A94903)

Name: Anti-POLR2A (phospho Ser1619) Antibody
Brand: Antibodies.com
SKU: A94903
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to POLR2A (phospho Ser1619) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-POLR2A (phospho Ser1619) Antibody

Description

Rabbit polyclonal antibody to POLR2A (phospho Ser1619).

Specificity

This antibody detects endogenous levels of POLR2A only when phosphorylated at Ser1619.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human POLR2A around the phosphorylation site of Ser1619 (amino acids 1585-1634).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

217kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, POLR2A, POLRA, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA polymerase II subunit 1, RNA polymerase II subunit B1, RP02, RPB220, RPBh1, RpIILS, RPO21, RPOL2, SUA8

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-POLR2A (phospho Ser1619) Antibody (A94903)

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' using Anti-POLR2A (phospho Ser1619) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-POLR2A (phospho Ser1619) Antibody (A94903)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-POLR2A (phospho Ser1619) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-POLR2A (phospho Ser1619) Antibody (A94903)

Immunofluorescence analysis of HeLa cells, treated with PMA 125ng/ml 30', using Anti-POLR2A (phospho Ser1619) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.