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Recombinant Anti-Poliovirus Receptor/PVR Antibody [DM146] - BSA and Azide free (A318562)

ELISA - Anti-Poliovirus Receptor/PVR Antibody [DM146] - Azide free (A318562) - Antibodies.com Flow Cytometry - Anti-Poliovirus Receptor/PVR Antibody [DM146] - Azide free (A318562) - Antibodies.com
Recombinant Antibody
$665
Product Datasheet

Recombinant rabbit monoclonal [DM146] antibody to Poliovirus Receptor/PVR for ELISA and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
7-8 Business Days

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Name
Anti-Poliovirus Receptor/PVR Antibody [DM146] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM146] antibody to Poliovirus Receptor/PVR.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM146
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Anti-Poliovirus Receptor/PVR Antibody [DM146] - Azide free (A318562) - Antibodies.comEnlarge Image
Figure 1: Anti-Poliovirus Receptor/PVR Antibody [DM146] - BSA and Azide free (A318562)
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human Poliovirus Receptor/PVR Protein (Fc Chimera 6xHis Tag) (A318381) can bind Anti-Poliovirus Receptor/PVR Antibody [DM146] - Azide free (A318562) in a linear range of 5-100 µg/ml.
Flow Cytometry - Anti-Poliovirus Receptor/PVR Antibody [DM146] - Azide free (A318562) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-Poliovirus Receptor/PVR Antibody [DM146] - BSA and Azide free (A318562)
CD155 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-Poliovirus Receptor/PVR Antibody [DM146] - Azide free (A318562) on Expi293 cells transfected with human CD155 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram), and isotype control on Expi293 transfected with irrelevant protein (orange histogram).
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