This antibody recognizes a glycosylation-dependent epitope (aa 207-210) on human PODXL, a highly glycosylated transmembrane glycoprotein expressed above all in many types of cancer tissues, and on embryonic stem cells.
Applications
WB, Flow Cytometry, IHC
Dilutions
Flow Cytometry: 1-4 µg/ml.
Reactivity
Human
Immunogen
Recombinant PODXL ectodomain.
Host
Mouse
Clonality
Monoclonal
Clone ID
PcMab-47
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of HeLa cells stained with Anti-PODXL Antibody [PcMab-47] (concentration in sample is 1.7 µg/ml, GAM-APC, red-filled) from HeLa cells unstained by primary antibody (GAM-APC, black-dashed) in flow cytometry analysis (surface staining).
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