Anti-PNPLA3 Antibody (A82487)

Name: Anti-PNPLA3 Antibody
Brand: Antibodies.com
SKU: A82487
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PNPLA3 for ELISA, WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PNPLA3 Antibody

Description

Goat polyclonal antibody to PNPLA3.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:16,000, WB: 0.01-0.03µg/ml, IHC: 8µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the internal region of mouse PNPLA3.

Sequence

EHDICPKVKSTN

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

45 kDa

Predicted MW

42.6Da

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, Calcium independent phospholipase A2 epsilon, DJ796I17.1, iPLA(2)epsilon, IPLA2-epsilon, Patatin like phospholipase domain containing 3, patatin like phospholipase domain containing protein 3, phospholipase A2, calcium independent, epsilon

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PNPLA3 Antibody (A82487)

PNPLA3 expression in Mouse Adrenal Gland lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PNPLA3 Antibody (A82487) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-PNPLA3 Antibody (A82487)

PNPLA3 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-PNPLA3 Antibody (A82487) at 8µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-PNPLA3 Antibody (A82487)

Negative control for PNPLA3 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 4: Immunofluorescence - Anti-PNPLA3 Antibody (A82487)

PNPLA3 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PNPLA3 Antibody (A82487) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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