Rabbit polyclonal antibody to PLK1 (Phospho-Ser137)
Specificity
The antibody detects endogenous levels of PLK1 only when phosphorylated at serine 137.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 137 (R-R-S(p)-L-L) derived from Human PLK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Jurkat cells treated with PMA, using PLK1 (Phospho-Ser137) antibody #12118. The lane on the right is treated with the synthesized peptide.
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