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Cardiovascular
Blood
Platelets
Anti-Pleckstrin Antibody (A83983)
This product is identical to Abcam (ab115514). Discover more for less.
Overview
Specifications
Images
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$480
Product Datasheet
Goat polyclonal antibody to Pleckstrin for ELISA, WB, IF, Flow Cytometry and IHC.
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Product Size:
100µg
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Shipping Information
Delivery
Freight Charges
$40 to United States
Lead Time
3-5 Business Days
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Specifications
Name
Anti-Pleckstrin Antibody
Description
Goat polyclonal antibody to Pleckstrin.
Applications
ELISA
,
WB
,
IF
,
Flow Cytometry
,
IHC
Dilutions
ELISA: 1:16,000, WB: 0.01-0.3µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human Pleckstrin.
Sequence
C-EWIKAIQMASRTGK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
40-45 kDa
Predicted MW
40.1kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
FLJ27168, OTTHUMP00000159962, p47, Platelet 47 kDa protein, Platelet and leukocyte C kinase substrate, Platelet P47, Platelet p47 protein, PLEK, PLEK_HUMAN
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (5)
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Figure 1: Western Blot - Anti-Pleckstrin Antibody (A83983)
Anti-PLEK Antibody (A83983) staining (0.01µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Figure 2: Immunohistochemistry - Anti-Pleckstrin Antibody (A83983)
Anti-PLEK Antibody (A83983) (3.75µg/ml) staining of paraffin embedded Human Vessel (platelets). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Figure 3: Immunohistochemistry - Anti-Pleckstrin Antibody (A83983)
Anti-PLEK Antibody (A83983) (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Figure 4: Immunofluorescence - Anti-Pleckstrin Antibody (A83983)
Anti-PLEK Antibody (A83983) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing cytoplasmic/membrane and nuclear staining.
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Figure 5: Flow Cytometry - Anti-Pleckstrin Antibody (A83983)
Anti-PLEK Antibody (A83983) - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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