Rabbit polyclonal antibody to PKC zeta (Phospho-Thr560)
Specificity
The antibody detects endogenous level of PKC zeta only when phosphorylated at threonine 560.
Applications
WB
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phospho rylation site of threonine560(Q-L-T(p)-P-D) derived from Human PKC zeta .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
nPKC-zeta, PKC2, PRKCZ, Protein kinase C zeta type
Western blot analysis of extracts from COS7 cells treated with PMA using Phospho-PKC zeta (Thr560) antibody #11958.The lane on the right is treated with the antigen-specific peptide.
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