Anti-PKC thet (phospho Ser695) Antibody (A94701)

Name: Anti-PKC thet (phospho Ser695) Antibody
Brand: Antibodies.com
SKU: A94701
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to PKC thet (phospho Ser695) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PKC thet (phospho Ser695) Antibody

Description

Rabbit polyclonal antibody to PKC thet (phospho Ser695).

Specificity

This antibody detects endogenous levels of PKC thet only when phosphorylated at Ser695.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:20000

Reactivity

Human

Immunogen

Synthetic peptide derived from human PKC thet around the phosphorylation site of Ser695 (amino acids 657-706).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

81kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

nPKC-theta, PRKCT, Protein kinase C theta, Protein kinase C theta type

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunohistochemistry - Anti-PKC thet (phospho Ser695) Antibody (A94701)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-PKC thet (phospho Ser695) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 2: Western Blot - Anti-PKC thet (phospho Ser695) Antibody (A94701)

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15' using Anti-PKC thet (phospho Ser695) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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