Anti-PKC delta Antibody [ARC1434] (A308143)

Name: Anti-PKC delta Antibody [ARC1434]
Brand: Antibodies.com
SKU: A308143
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1434] antibody to PKC delta for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PKC delta Antibody [ARC1434]

Description

Rabbit monoclonal [ARC1434] antibody to PKC delta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 547-653 of human PKC delta (Q05655).

Sequence

PFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLID

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1434

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CVID9, D14Ertd420e, Kinase PKC delta, KPCD, KPCD_HUMAN, MAY 1, MAY1, MGC49908, nPKC delta, nPKC-delta, PCKd, PKC d, PKCD, PKCdelta, PRKC D, PRKC delta, Prkcd, Protein Kinase C delta, Protein kinase C delta type, Protein kinase C delta VIII, Protein Kinase Cdelta, Tyrosine protein kinase PRKCD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PKC delta Antibody [ARC1434] (A308143)

Western blot analysis of extracts of various cell lines, using Anti-PKC delta Antibody [ARC1434] (A308143) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-PKC delta Antibody [ARC1434] (A308143)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-PKC delta Antibody [ARC1434] (A308143) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PKC delta Antibody [ARC1434] (A308143)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-PKC delta Antibody [ARC1434] (A308143) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-PKC delta Antibody [ARC1434] (A308143)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PKC delta Antibody [ARC1434] (A308143) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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