Western Blot - Anti-PKC alpha (phospho Thr638) Antibody [ARC58020] (A309441)
Western blot analysis of 293T, using Anti-PKC alpha (phospho Thr638) Antibody [ARC58020] (A309441) at 1:7,000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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