PKA alpha + beta (catalytic subunits) (phospho Thr197) Cell Based ELISA Kit (A102523)

$645
Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
PKA alpha + beta (catalytic subunits) (phospho Thr197) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This PKA alpha + beta (catalytic subunits) (phospho Thr197) Cell Based ELISA Kit allows for the detection of PKA alpha + beta (catalytic subunits) (phospho Thr197) and the effects that certain stimulation conditions have on PKA alpha + beta (catalytic subunits) (phospho Thr197) expression in different cell lines. Qualitative determination of PKA alpha + beta (catalytic subunits) (phospho Thr197) concentration is achieved by an indirect ELISA format. In essence, the PKA alpha + beta (catalytic subunits) (phospho Thr197) is captured by Anti-PKA alpha + beta (catalytic subunits) (phospho Thr197) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PKA alpha + beta (catalytic subunits) (phospho Thr197) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-PKA alpha + beta (catalytic subunits) Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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