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Anti-PIGM Antibody (A309211)

Western Blot - Anti-PIGM Antibody (A309211) - Antibodies.com Immunohistochemistry - Anti-PIGM Antibody (A309211) - Antibodies.com Immunohistochemistry - Anti-PIGM Antibody (A309211) - Antibodies.com Immunofluorescence - Anti-PIGM Antibody (A309211) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to PIGM for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-PIGM Antibody
Description
Rabbit polyclonal antibody to PIGM.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 35-96 of human PIGM (NP_660150.1).
Sequence
YGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
49 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
dol P Man dependent GPI mannosyltransferase, DPM GlcN (acyl)PI mannosyltransferase, GPI mannosyltransferase 1, GPI mannosyltransferase I, GPI MT I, Phosphatidylinositol glycan anchor biosynthesis, class M, Phosphatidylinositol glycan biosynthesis class M protein, PIG M mannosyltransferase
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PIGM Antibody (A309211) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PIGM Antibody (A309211)
Western blot analysis of extracts of various cell lines, using Anti-PIGM Antibody (A309211) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.
Immunohistochemistry - Anti-PIGM Antibody (A309211) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-PIGM Antibody (A309211)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-PIGM Antibody (A309211) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PIGM Antibody (A309211) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-PIGM Antibody (A309211)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PIGM Antibody (A309211) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-PIGM Antibody (A309211) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-PIGM Antibody (A309211)
Immunofluorescence analysis of L929 cells using Anti-PIGM Antibody (A309211) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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