Anti-PIAS3 Antibody (A16891)

Name: Anti-PIAS3 Antibody
Brand: Antibodies.com
SKU: A16891
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PIAS3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-PIAS3 Antibody

Description

Rabbit polyclonal antibody to PIAS3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 452-628 of human PIAS3 (NP_006090.2).

Sequence

IESSSDEEDLPPTKKHCSVTSAAIPALPGSKGVLTSGHQPSSVLRSPAMGTLGGDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHFFQYRGTPSHFLGPLAPTLGSSHCSATPAPPPGRVSSIVAPGGALREGHGGPLPSGPSLTGCRSDIISLD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 - 75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 SUMO protein ligase PIAS 3, E3 SUMO protein ligase PIAS3, E3 SUMO-protein ligase PIAS3, FLJ14651, OTTHUMP00000015586, OTTHUMP00000015587, PIAS 3, PIAS3 protein, PIAS3_HUMAN, Protein inhibitor of activated STAT 3, Protein inhibitor of activated STAT protein 3, Protein inhibitor of activated STAT3, Zinc finger MIZ type containing 5, ZMIZ 5, ZMIZ5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PIAS3 Antibody (A16891)

Western blot analysis of 293F, using Anti-PIAS3 Antibody (A16891) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-PIAS3 Antibody (A16891)

Western blot analysis of Mouse thymus, using Anti-PIAS3 Antibody (A16891) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-PIAS3 Antibody (A16891)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PIAS3 Antibody (A16891) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PIAS3 Antibody (A16891)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-PIAS3 Antibody (A16891) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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