Anti-Phosphotyrosine Antibody [PY20] (HRP) (A281610)

$665

Mouse monoclonal [PY20] antibody to Phosphotyrosine (HRP) for ELISA.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Phosphotyrosine Antibody [PY20] (HRP)
Description
Mouse monoclonal [PY20] antibody to Phosphotyrosine (HRP).
Specificity
This antibody recognises phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Applications
ELISA
Dilutions
ELISA: 1:1,000 - 1:4,000
Reactivity
Species Independent
Host
Mouse
Clonality
Monoclonal
Clone ID
PY20
Isotype
IgG2b
Conjugate

HRP

Purification
Affinity chromatography of ascites.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.01% Thiomersal and 50% Glycerol.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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