Anti-Phosphotyrosine Antibody [PY20] (HRP) (A281610)

Anti-Phosphotyrosine Antibody [PY20] (HRP)

Mouse monoclonal [PY20] antibody to Phosphotyrosine (HRP).

This antibody recognises phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.

ELISA: 1:1,000 - 1:4,000

Species Independent

Mouse

Monoclonal

PY20

HRP

Affinity chromatography of ascites.

Lot Specific

Liquid

Supplied in Phosphate Buffered Saline with 0.01% Thiomersal and 50% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.

This product is for research use only. It is not intended for diagnostic or therapeutic use.