Mouse monoclonal [G104] antibody to Phosphotyrosine.
Specificity
This antibody recognises phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:1,000, IHC: 1:100
Reactivity
Species Independent
Cross Reactivity
This antibody does not cross-react with phosphoserine or phosphothreonine.
Immunogen
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide.
Host
Mouse
Clonality
Monoclonal
Clone ID
G104
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1: Western Blot - Anti-Phosphotyrosine Antibody [G104] (A304807)
Western blot analysis of human A431 cell lysates showing detection of Phosphotyrosine protein using Anti-Phosphotyrosine Antibody [G104] (A304807) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature. Left: normal, right: EGF treated.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-Phosphotyrosine Antibody [G104] (A304807) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Stratum granulosum staining in the epidermis. Some dermal staining.
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