Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Phosphothreonine Antibody (A304810), at 1:60 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.
Figure 2: Western Blot - Anti-Phosphothreonine Antibody (A304810)
Western blot analysis of mouse brain cell lysates showing detection of Phosphothreonine protein using Anti-Phosphothreonine Antibody (A304810) at 1:1,000. Left: Treated with Vanadium, Right: Non-treated..
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Phosphothreonine Antibody (A304810), at 1:60 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.
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