Anti-PHIP Antibody (A12092)

Name: Anti-PHIP Antibody
Brand: Antibodies.com
SKU: A12092
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PHIP for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PHIP Antibody

Description

Rabbit polyclonal antibody to PHIP.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1542-1821 of human PHIP (NP_060404.3).

Sequence

KTILENSVKHSKALNTLSSPGQSSFSHGTRNNSAKENMEKEKPVKRKMKSSVLPKASTLSKSSAVIEQGDCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDLLQKKNRGGRKPKRKMKTQKLDADLLVPASVKVLRRSNRKKIDDPIDEEEEFEELKGSEPHMRTRNQGRRTAFYNEDDSEEEQRQLLFEDTSLTFGTSSRGRVRKLTEKAKANLIGW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DCAF14, DDB1 and CUL4 associated factor 14, FLJ20705, FLJ45918, IRS 1 PH domain binding protein, IRS1 PH domain binding protein, MGC90216, Ndrp, Neuronal differentiation related protein, PH interacting protein, Pleckstrin homology domain interacting protein, WD repeat containing protein 11, WD repeat protein 11, WDR11

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PHIP Antibody (A12092)

Western blot analysis of extracts of various cell lines, using Anti-PHIP Antibody (A12092) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-PHIP Antibody (A12092)

Immunohistochemistry analysis of paraffin-embedded human well-differentiated squamous skin carcinoma using Anti-PHIP Antibody (A12092) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PHIP Antibody (A12092)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using Anti-PHIP Antibody (A12092) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PHIP Antibody (A12092)

Immunohistochemistry analysis of paraffin-embedded mouse tumor using Anti-PHIP Antibody (A12092) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.