Anti-PGC1 alpha Antibody (A84356)

Name: Anti-PGC1 alpha Antibody
Brand: Antibodies.com
SKU: A84356
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to PGC1 alpha for ELISA, IF, IHC and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PGC1 alpha Antibody

Description

Goat polyclonal antibody to PGC1 alpha.

Applications

ELISA, IF, IHC, Flow Cytometry

Dilutions

ELISA: 1:2,000, WB: 0.3-1µg/ml, IHC: 6-8µg/ml, IF: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human PGC1 alpha.

Sequence

C-DGLFDDSEDESDK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

90 kDa

Predicted MW

91.0kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

L PGC 1alpha, LEM6, Ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC 1 (alpha), PGC 1 alpha, PGC 1v, PGC-1-alpha, PGC1, PGC1(alpha), PGC1A, PGC1v, PPAR gamma coactivator 1, PPAR gamma coactivator 1 alpha, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, PPAR gamma coactivator variant form, PPAR-gamma coactivator 1-alpha, PPARGC 1 alpha, PPARGC-1-alpha, PPARGC1, PPARGC1A, PRGC1_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Immunofluorescence - Anti-PGC1 alpha Antibody (A84356)

PGC1 alpha expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A84356) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-PGC1 alpha Antibody (A84356)

PGC1 alpha expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A84356) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-PGC1 alpha Antibody (A84356)

PGC1 alpha expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A84356) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-PGC1 alpha Antibody (A84356)

PGC1 alpha expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-PGC1 alpha Antibody (A84356) at 6µg/ml and revealed with horseradish peroxidase (HRP).