Anti-PFKFB3 Antibody (A15442)

Name: Anti-PFKFB3 Antibody
Brand: Antibodies.com
SKU: A15442
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PFKFB3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-PFKFB3 Antibody

Description

Rabbit polyclonal antibody to PFKFB3.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human PFKFB3 (NP_004557.1).

Sequence

TRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase, 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3, 6-bisphosphatase, 6-P2ase 3, 6-P2ASE brain/placenta-type isozyme, 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme, 6PF 2-K/Fru 2,6 P2ase 3, 6PF-2-K/Fru-2, F263_HUMAN, fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase, Fructose-2, Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase, iPFK 2, iPFK-2, IPFK2, PFK/FBPase 3, PFK2, Renal carcinoma antigen NY REN 56, Renal carcinoma antigen NY-REN-56, uPFK 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PFKFB3 Antibody (A15442)

Western blot analysis of extracts of various cell lines, using Anti-PFKFB3 Antibody (A15442) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-PFKFB3 Antibody (A15442)

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Figure 3: Immunohistochemistry - Anti-PFKFB3 Antibody (A15442)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-PFKFB3 Antibody (A15442) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PFKFB3 Antibody (A15442)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-PFKFB3 Antibody (A15442) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.