Mixed neuron/glia cultures from newborn rat brain stained with Anti-Peripherin Antibody (green) and Anti-NF-H Antibody (A85337 | red). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.
Immunofluorescent analysis of the widely used rat pheochromocytoma PC12 cell line stained with Anti-Peripherin Antibody (A85430), at a dilution of 1:2,000, in green. The nuclear DNA is visualised in blue using Hoechst staining. Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell, forming a perinuclear cap with some filaments visible in the cytoplasm.
Figure 3: Western Blot - Anti-Peripherin Antibody (A85430)
Western blot analysis of tissue and cell lysates using Anti-Peripherin Antibody (A85430), at a dilution of 1:10,000 in green. The lanes contain samples of: [1] Protein standards, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at approximately 57 kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.
Figure 4: Western Blot - Anti-Peripherin Antibody (A85430)
Western blot of whole rat spinal cord homogenate stained with Anti-Peripherin Antibody (1:1,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~57 kDa corresponds to Peripherin.
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