Anti-Perilipin-1 Antibody [ARC1122] (A306256)

Name: Anti-Perilipin-1 Antibody [ARC1122]
Brand: Antibodies.com
SKU: A306256
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1122] antibody to Perilipin-1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Perilipin-1 Antibody [ARC1122]

Description

Rabbit monoclonal [ARC1122] antibody to Perilipin-1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Perilipin A (O60240).

Sequence

HYVPLPRLSLMEPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRVSDSF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1122

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Lipid droplet associated protein, Lipid droplet-associated protein, PERI, Perilipin, Perilipin A, PerilipinA, PLIN, PLIN1, PLIN1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Perilipin-1 Antibody [ARC1122] (A306256)

Western blot analysis of extracts of various cell lines, using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Perilipin-1 Antibody [ARC1122] (A306256)

Immunohistochemistry analysis of paraffin-embedded rat brown adipose using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Perilipin-1 Antibody [ARC1122] (A306256)

Immunohistochemistry analysis of paraffin-embedded mouse brown adipose using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Perilipin-1 Antibody [ARC1122] (A306256)

Immunofluorescence analysis of rat fat using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).