This antibody recognises perforin, also known as cytolysin, a cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. The importance of perforin in maintaining immune homeostasis and immune surveillance is extremely well documented. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis
Applications
WB
Dilutions
WB: 0.5 - 4.0 µg/ml
Reactivity
Rat, Human, Mouse
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 568 of rat perforin.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
500 µg/ml
Molecular Weight
Approximately 70 kDa in Jurkat lysates
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 30% Glycerol, 0.5% BSA, and 0.01% Thiomersal.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti perforin antibody recognizes perforin, also known as cytolysin, acytolyticprotein found in the granules ofcytotoxic T lymphocytes (CTLs)andNK cells. The importance of perforin in maintaining immune homeostasis and immune surveillance is extremely well documented. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis