Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at 1:1,000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of C6 cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of NIH-3T3 cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of U-2 OS cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).