Anti-PDHA2 Antibody (A12424)

Name: Anti-PDHA2 Antibody
Brand: Antibodies.com
SKU: A12424
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PDHA2 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PDHA2 Antibody

Description

Rabbit polyclonal antibody to PDHA2.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1).

Sequence

HGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 1.2.4.1, MGC149517, MGC149518, mitochondrial, ODPAT_HUMAN, PDHAL, PDHE1 A type II, PDHE1-A type II, Pyruvate dehydrogenase (lipoamide) alpha 2, Pyruvate dehydrogenase E1 component subunit alpha, Pyruvate dehydrogenase E1 component subunit alpha, testis specific form, mitochondrial, Pyruvate dehydrogenase, E1 alpha polypeptide, testis spec, testis-specific form

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PDHA2 Antibody (A12424)

Western blot analysis of extracts of various cell lines, using Anti-PDHA2 Antibody (A12424) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-PDHA2 Antibody (A12424)

Immunofluorescence analysis of C6 cells using Anti-PDHA2 Antibody (A12424) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-PDHA2 Antibody (A12424)

Immunofluorescence analysis of HeLa cells using Anti-PDHA2 Antibody (A12424) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PDHA2 Antibody (A12424)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PDHA2 Antibody (A12424) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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