Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Name: Anti-PDHA1 (phospho Ser293) Antibody [ARC53489]
Brand: Antibodies.com
SKU: A306031
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC53489] antibody to PDHA1 (phospho Ser293) for WB, ICC/IF and IP.

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$40 to United States

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5-8 Business Days

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Specifications

Name

Anti-PDHA1 (phospho Ser293) Antibody [ARC53489]

Description

Rabbit monoclonal [ARC53489] antibody to PDHA1 (phospho Ser293).

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:2,000-1:20,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).

Sequence

GHSMS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53489

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

mitochondrial, ODPA_HUMAN, PDH, PDHA, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate dehydrogenase complex E1 alpha polypeptide 1, Pyruvate dehydrogenase complex, E1 alpha polypeptide 1, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, Pyruvate Dehydrogenase E1-alpha subunit, Pyruvate dehydrogenase, alpha-1, somatic form

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of various cell lines, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:1,000 dilution. Hela cells and NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of HeLa cells, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:115000 dilution. 293T cells were treated by Î»-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of HeLa cells, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:20,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunofluorescence - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Immunofluorescence analysis of NIH/3T3 using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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A89894

Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Rabbit monoclonal [ARC53489] antibody to PDHA1 (phospho Ser293) for WB, ICC/IF and IP.

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