Western Blot - Anti-PDGFR beta (phospho Tyr771) Antibody (A308748)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-PDGFR beta (phospho Tyr771) Antibody (A308748) at 1:1,000 dilution or Phospho-PDGFRB-Y771 antibody (A2180). NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
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